Peer Reviewed Journal
2025, Vol. 5, Issue 2, Part A
Development and characterization of flavonosome formulation loaded with hesperidine and berberine
Author(s): Rishabh Tiwari, Munesh Singh Bhadouria and Avinash K Kondalkar
Abstract: The present investigation aimed to develop and comprehensively characterize a novel flavonosome drug delivery system co-loaded with hesperidine and berberine to overcome the inherent bioavailability limitations of these bioactive flavonoids. Extensive pre-formulation studies were conducted to establish the physicochemical properties of both compounds, including organoleptic characteristics, solubility profiles, and analytical method development using UV- Visible spectrophotometry. Flavonosomes were successfully prepared using a bulk co-loading method employing lecithin as the phospholipid carrier. Six different formulations (F1-F6) were prepared and systematically evaluated for particle size, polydispersity index, surface morphology, entrapment efficiency, and in vitro release characteristics. The optimized formulation F4 demonstrated superior characteristics with a particle size of 178.3 nm, polydispersity index of 0.421, and remarkable entrapment efficiency of 88.08%. Differential Scanning Calorimetry (DSC) confirmed successful complex formation through molecular interactions between flavonoids and phospholipids. In vitro release studies revealed sustained release behavior with 96.05% release for hesperidine and 91.22% for berberine over 12 hours. Stability studies conducted according to ICH guidelines demonstrated excellent physical stability. Antioxidant evaluation using DPPH radical scavenging assay showed enhanced antioxidant activity of the flavonosome formulation (IC₅₀ = 151.6 μg/mL) compared to individual compounds (hesperidine IC₅₀ = 168.99 μg/mL, berberine IC₅₀ = 220.44 μg/mL). This study conclusively demonstrates the potential of flavonosome-based delivery systems as an effective strategy to improve the therapeutic efficacy and bioavailability of naturally derived bioactive compounds.
Pages: 55-60 | Views: 93 | Downloads: 57
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How to cite this article:
Rishabh Tiwari, Munesh Singh Bhadouria, Avinash K Kondalkar. Development and characterization of flavonosome formulation loaded with hesperidine and berberine. Nat J Pharm Sci 2025;5(2):55-60.
