National Journal of Pharmaceutical Sciences
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P-ISSN: 2788-9262, E-ISSN: 2788-9270

2023, Vol. 3, Issue 2, Part A


Stability indicating method development and validation of sumatriptan by using RP–HPLC method and its dosage form


Author(s): Chaithanya A, Paul Richards M and BA Vishwanath

Abstract: This is a method has established to stability indicating method development and validation for sumatriptan by Reverse Phase-HPLC method. Under chromatographic conditions the Phenomenex kinetex (250*4.6 mm, 5µi.d) column has been used and 1 ml/min flow rate has been maintained, The Acetonitrile 10% Methanol 10% and Tri ethyl amine 80% (adjusted pH along with orthophosphoric acid) of mobile phase has taken. So that 221 nm of wavelength was detected. A simple, precise, accurate and rapid high performance liquid chromatographic method has been developed and validated for the estimation of sumatriptan in tablet dosage forms. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 800 ng/spot. The limit of detection and the limit of quantification for the sumatriptan were found to be 63.87 and 193.54 ng, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.

Pages: 19-27 | Views: 806 | Downloads: 432

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National Journal of Pharmaceutical Sciences
How to cite this article:
Chaithanya A, Paul Richards M, BA Vishwanath. Stability indicating method development and validation of sumatriptan by using RP–HPLC method and its dosage form. Nat J Pharm Sci 2023;3(2):19-27.


National Journal of Pharmaceutical Sciences

National Journal of Pharmaceutical Sciences

National Journal of Pharmaceutical Sciences
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