2023, Vol. 3, Issue 1, Part B
Evaluation of in vitro antiplasmodial, anti-inflammatory activities and in vivo oral acute toxicity of Trichoscypha acuminata Engl (Anacardiaceae) stem bark
Author(s): Jean Emmanuel MbossoTeinkela, Lisette Pulchérie Yoyo Ngando, Hans Denis Bamal, Eugène Ekounè Kamè, Albert Emmanuel Mpondo Mpondo, Jules Clément Assob Nguedia and Simone Véronique Fannang
Abstract: The promotion of traditional medicine by regulating the search for a new adequate therapeutic response and a low price is one of the objectives of the WHO strategy for the years 2014-2023. The genus Trichoscypha of the family Anacardiaceae is used in traditional medicine to treat bronchial diseases, rheumatic diseases, sterility, anemia and cancer. Therefore, our work focused on the evaluation of the antiplasmodial, of the total extract and resulted fractions from Trichoscypha acuminata Engl (Anacardiaceae) stem bark, as well as the anti-inflammatory activities and the acute oral toxicity of the total extract.The in vitro cell-growth inhibition activities were assessed against Plasmodium falciparum strain 3D7 for the antiplasmodial activity. The anti-inflammatory activity was tested only on the total extract (TETAsb) by quantification of the percentage of denaturation of hen egg albumin with (HEA) a concentration range of 25 to 1600 μg/mL of tested extract. while the in vivo acute oral toxicity was determined according to the modified Organisation for Economic Co-operation and Development (OECD) guidelines 423 at 2000 mg/kg and 5000 mg/kg body weight on female Wistar strain laboratory rats.Phytochemical screening revealed the presence of tannins, phenols, triterpenes, flavonoids, alkaloids and glycosides. The antiplasmodial activity showed that only the HFTAsb and DFTAsb fractions at a concentration of 20 μg/mL reduced the viability of P. falciparum 3D7 cellswith viability percentages of 39.43% and 40.61%, respectively, and IC50 of 29.90 and 28.16 µg/mL, respectively. The total MeOH extract (TETAsb) showed greater anti-inflammatory activity than the reference molecule Diclofenac with a maximum of 428.33% following the HEA denaturation test. An oral administration of the aqueous fraction did not induce an abnormal variation of the physiological parameters in female Wistar laboratory rats, at non-toxic doses up to 5000 mg/kg body weight for 14 days. These results confirm the use of this plant in traditional medicine to treat rheumatic diseases. These studies are the first biological studies conducted on the genus Trichoscypha and the species Trichoscypha acuminata. All these results constitute a biological basis of this species and this genus.
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How to cite this article:
Jean Emmanuel MbossoTeinkela, Lisette Pulchérie Yoyo Ngando, Hans Denis Bamal, Eugène Ekounè Kamè, Albert Emmanuel Mpondo Mpondo, Jules Clément Assob Nguedia, Simone Véronique Fannang. Evaluation of in vitro antiplasmodial, anti-inflammatory activities and in vivo oral acute toxicity of Trichoscypha acuminata Engl (Anacardiaceae) stem bark. Nat J Pharm Sci 2023;3(1):107-114.
